Coding

Part:BBa_K3941001:Design

Designed by: Begüm Esra Aytan   Group: iGEM21_Saint_Joseph   (2021-07-26)


EGII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created from only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag has added to 3' end of the sequence to help purification of protein product.


Source

The source of this part is Trichoderma reesei endoglucanase II gene.

References